Seq-Well: A Sample-Efficient, Portable Picowell Platform for Massively Parallel Single-Cell RNA Sequencing

  • R&D
  • Technology
  • Toby Aicher
  • Shaina Carroll
  • Marc Wadsworth
  • Travis Hughes
  • Alex K. Shalek
  • Aicher et al.▾
    Aicher, T.P., Carroll, S., Raddi, G., Gierahn, T., Wadsworth, M.H. II, Hughes, T.K., Love, C., Shalek, A.K.
  • Single-Cell Methods
  • May, 2019
R&D
Technology
Toby Aicher
Shaina Carroll
Marc Wadsworth
Travis Hughes
Alex K. Shalek

Abstract

Seq-Well is a low-cost picowell platform that can be used to simultaneously profile the transcriptomes of thousands of cells from diverse, low input clinical samples. In Seq-Well, uniquely barcoded mRNA capture beads and cells are co-confined in picowells that are sealed using a semipermeable membrane, enabling efficient cell lysis and mRNA capture. The beads are subsequently removed and processed in parallel for sequencing, with each transcript’s cell of origin determined via the unique barcodes. Due to its simplicity and portability, Seq-Well can be performed almost anywhere.

Seq-Well: A Sample-Efficient, Portable Picowell Platform for Massively Parallel Single-Cell RNA Sequencing