Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput

  • Chemistry
  • Genomics
  • R&D
  • Technology
  • Travel
  • Marc Wadsworth
  • Travis Hughes
  • Alex K. Shalek
  • Gierahn et al.▾
    Gierahn, T.M*., Wadsworth II, M.H.*, Hughes, T.K.*, Bryson, B.D., Butler, A., Satija, R., Fortune, S., Love, J.C.†, Shalek, A.K.†
  • Nature Methods , Volume 14
  • March, 2017
Chemistry
Genomics
R&D
Technology
Travel
Marc Wadsworth
Travis Hughes
Alex K. Shalek

Abstract

Single-cell RNA-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA-seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We use Seq-Well to profile thousands of primary human macrophages exposed to Mycobacterium tuberculosis.

Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput