Induction of metabolic quiescence defines the transitional to follicular B cell switch
Biology
Genomics
Immunology
Toby Aicher
Alex K. Shalek
The next quarter century
BiologyGenomicsImmunologyAlex K. Shalek
Germline-encoded affinity for cognate antigen enables vaccine amplification of a human broadly neutralizing response against influenza virus
BiologyGenomicsImmunology
Infectious Disease
Medicine
Sam KazerAlex K. Shalek
Loss of DNA methyltransferase activity in primed human ES cells triggers increased cell-cell variability and transcriptional repression
BiologyGenomics
Marc Wadsworth II
Sam Allon
Ben Mead
Riley DrakeAlex K. Shalek
Highly efficient, massively-parallel single-cell RNA-Seq reveals cellular states and molecular features of human skin pathology
BiologyGenomicsImmunology
R&D
Technology
Travis HughesMarc Wadsworth II
José Ordovas-MontañesAlex K. Shalek
Integrated Single-Cell Analysis of Multicellular Immune Dynamics during Hyper-Acute HIV-1 Infection
BiologyGenomicsImmunologyInfectious DiseaseSam KazerToby Aicher
Shaina CarrollJosé Ordovas-Montañes
Carly Ziegler
Sarah NyquistAlex K. ShalekSeq-Well: A Sample-Efficient, Portable Picowell Platform for Massively Parallel Single-Cell RNA Sequencing
R&DTechnologyToby AicherShaina CarrollMarc Wadsworth IITravis HughesAlex K. Shalek
Mechanisms of lymphoma clearance induced by high-dose alkylating agents
Biology
CancerImmunology
Kellie Kolb
Jay PrakadanAlex K. Shalek
Single-Cell Analysis of the Liver Epithelium Reveals Dynamic Heterogeneity and an Essential Role for YAP in Homeostasis and Regeneration
Biology
Cell AtlasMedicineJosé Ordovas-MontañesAlex K. Shalek
Single-Cell RNA-Seq Reveals AML Hierarchies Relevant to Disease Progression and Immunity
Marc Wadsworth IITravis HughesAlex K. Shalek
Optofluidic real-time cell sorter for longitudinal CTC studies in mouse models of cancer
BiologyCancer
Computational MethodsR&DTechnologyJay Prakadan
Andrew Navia
Peter WinterRiley Drake
Alejandro GuptaAlex K. Shalek
Linking single-cell measurements of mass, growth rate, and gene expression
BiologyCancerGenomics
PhysicsR&DTechnologyJay PrakadanAlejandro GuptaRiley DrakeAlex K. Shalek
Selective expansion of myeloid and NK cells in humanized mice yields human-like vaccine responses
BiologyGenomicsImmunologyInfectious DiseaseAlex K. ShalekCarly Ziegler
T Helper Cell Cytokines Modulate Intestinal Stem Cell Renewal and Differentiation
GenomicsImmunologyMedicineJosé Ordovas-MontañesAlex K. Shalek
High-Frequency, Functional HIV-Specific T-Follicular Helper and Regulatory Cells Are Present Within Germinal Centers in Children but Not Adults
Toby AicherSam KazerAlex K. Shalek
Allergic Inflammatory Memory in Human Respiratory Epithelial Progenitor Cells
BiologyCell AtlasImmunologyMedicineJosé Ordovas-MontañesSarah Nyquist
Marko VukovicMarc Wadsworth IITravis HughesSam KazerAlex K. Shalek
Harnessing single-cell genomics to improve the physiological fidelity of organoid-derived cell types
Ben MeadJosé Ordovas-MontañesTravis HughesMarc Wadsworth IIAlex K. Shalek
Linking single-cell measurements of mass, growth rate, and gene expression
ChemistryGenomicsTechnologyJay PrakadanAlejandro GuptaRiley DrakeAlex K. Shalek
Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells
GenomicsImmunologyAndrew NaviaAlex K. Shalek
Science Forum: The Human Cell Atlas
BiologyCell AtlasComputational MethodsGenomicsImmunologyMedicineTechnologyAlex K. Shalek
Single-cell analyses to tailor treatments
ImmunologyInfectious DiseaseMedicineTechnologyAlex K. Shalek
Novel in vitro booster vaccination to rapidly generate antigen-specific human monoclonal antibodies
GenomicsImmunologyInfectious DiseaseMedicine
MicrobiologySam KazerJosé Ordovas-MontañesAlex K. Shalek
Scaling by shrinking: empowering single-cell ‘omics’ with microfluidic devices
GenomicsTechnologyJay PrakadanAlex K. Shalek
Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput
ChemistryGenomicsR&DTechnology
TravelMarc Wadsworth IITravis HughesAlex K. Shalek
Antiviral CD8+ T Cells Restricted by Human Leukocyte Antigen Class II Exist during Natural HIV Infection and Exhibit Clonal Expansion
BiologyImmunologyInfectious DiseaseSam KazerAlex K. Shalek
Regulation of X-linked gene expression during early mouse development by Rlim
BiologyGenomicsAlex K. Shalek
Multiplexed, targeted profiling of single-cell proteomes and transcriptomes in a single reaction
BiologyChemistryGenomicsR&DTechnology
Alex GenshaftJay PrakadanCarly ZieglerAlex K. Shalek
Dissecting the multicellular ecosystem of metastatic melanoma by single-cell RNA-seq
BiologyCancerGenomicsMedicineJay PrakadanMarc Wadsworth IIAlex GenshaftTravis HughesCarly ZieglerSam Kazer
Aleth GaillardKellie KolbAlex K. Shalek
Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM
ChemistryGenomicsTechnologySam KazerAleth GaillardAlex K. Shalek
Innate Lymphoid Cells Are Depleted Irreversibly during Acute HIV-1 Infection in the Absence of Viral Suppression
BiologyGenomicsImmunologyInfectious DiseaseSam KazerAlex K. Shalek
A microfluidic platform enabling single-cell RNA-seq of multigenerational lineages
BiologyGenomicsImmunologyR&DTechnologyAlex GenshaftSam KazerAlex K. Shalek
Single-Cell Genomics Unveils Critical Regulators of Th17 Cell Pathogenicity
BiologyGenomicsImmunologyAlex K. Shalek
Oct1 and OCA-B are selectively required for CD4 memory T cell function
BiologyGenomicsImmunologyAlex K. Shalek
Pathogen Cell-to-Cell Variability Drives Heterogeneity in Host Immune Responses
BiologyGenomicsImmunologyInfectious DiseaseMicrobiologyTechnologyAlex K. Shalek
Marrying microfluidics and microwells for parallel, high-throughput single-cell genomics
GenomicsR&DTechnologyMarc Wadsworth IITravis HughesAlex K. Shalek
Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets
GenomicsR&DTechnologyAlex K. Shalek
Deconstructing transcriptional heterogeneity in pluripotent stem cells
BiologyGenomicsAlex K. Shalek
Somatic mutation as a mechanism of Wnt/β-catenin pathway activation in CLL
CancerGenomicsAlex K. Shalek
Single-cell RNA-seq reveals dynamic paracrine control of cellular variation
BiologyGenomicsAlex K. Shalek
Single-cell RNA-seq highlights intratumoral heterogeneity in primary glioblastoma
CancerGenomicsAlex K. Shalek
Heterogeneity in immune responses: from populations to single cells
GenomicsImmunologyTechnologyAlex K. Shalek
Reconstructing and Reprogramming the Tumor-Propagating Potential of Glioblastoma Stem-like Cells
BiologyCancerGenomicsAlex K. Shalek
Whole-exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer
BiologyCancerGenomicsAlex K. Shalek
Single-cell transcriptomics reveals bimodality in expression and splicing in immune cells
BiologyGenomicsImmunologyAlex K. Shalek
Transcriptional and Epigenetic Dynamics during Specification of Human Embryonic Stem Cells
BiologyGenomicsAlex K. Shalek
Dynamic regulatory network controlling TH17 cell differentiation
BiologyGenomicsImmunologyAlex K. Shalek
Probing Enzymatic Activity inside Living Cells Using a Nanowire−Cell “Sandwich” Assay
BiologyChemistryImmunologyTechnologyAlex K. Shalek
Nanowire-Mediated Delivery Enables Functional Interrogation of Primary Immune Cells: Application to the Analysis of Chronic Lymphocytic Leukemia
CancerGenomicsR&DTechnologyAlex K. Shalek
Systematic Discovery of TLR Signaling Components Delineates Viral-Sensing Circuits
GenomicsImmunologyInfectious DiseaseAlex K. Shalek
Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells
ChemistryGenomicsPhysicsR&DTechnologyAlex K. Shalek