Biography

Constantine Tzouanas is a Hertz Fellow and NSF Graduate Research Fellow in the Harvard-MIT Health Sciences and Technology program, pursuing a PhD in Medical Engineering and Medical Physics with a concentration in Biological Engineering.

In the Shalek lab, he researches how the properties and interactions of individual cells produce the emergent phenomena needed for tissue/organism-level function, but go awry in disease states. His work combines technology development, bioinformatics, and wet lab experimentation to bring hard engineering rigor to the squishy world of biology.

As an undergraduate at Rice University, he majored in Bioengineering and minored in Neuroscience. He received the Goldwater Scholarship and worked on circuit neuroscience in the lab of Prof. Jacob Robinson, developing microfluidic devices to uncover principles of neural plasticity in the immortal jellyfish Hydra vulgaris.

Outside of research and coursework at MIT and Harvard, Constantine serves as an officer of the MIT Biotech Group and in the Harvard-MIT Health Sciences and Technology Joint Council. In his free time, Constantine enjoys running, ultimate frisbee, and baking. As mildly interesting trivia facts, his current favorite upbeat indie band is Saint Motel, and his childhood dream job was to become a skyscraper architect.

Single-cell RNA sequencing of liver fine-needle aspirates captures immune diversity in the blood and liver in chronic hepatitis B patients
  • Genshaft et al.,
  • Hepatology,
  • 2023
  • Technology
  • Cell Atlas
  • Biology
  • Immunology
  • R&D
  • Genomics
  • Alex Genshaft
  • Mike Vilme
  • Riley Drake
  • Ira Fleming
  • Nancy Tran
  • Constantine Tzouanas
  • Jasmin Joseph-Chazan
  • Alex K. Shalek
Multimodal profiling of lung granulomas in macaques reveals cellular correlates of tuberculosis control
  • Gideon et al.,
  • Immunity,
  • 2022
  • Microbiology
  • Infectious Disease
  • Genomics
  • Biology
  • Immunology
  • Cell Atlas
  • Travis Hughes
  • Constantine Tzouanas
  • Marc Wadsworth II
  • Conner Kummerlowe
  • Sarah Nyquist
  • Samira Ibrahim
  • José Ordovas-Montañes
  • Alex K. Shalek
Clinical implementation of single-cell RNA sequencing using liver fine needle aspirate tissue sampling and centralized processing captures compartment specific immuno-diversity
  • Genshaft et al.,
  • bioRxiv,
  • 2021
  • Technology
  • Immunology
  • Computational Methods
  • Genomics
  • Infectious Disease
  • Biology
  • Cell Atlas
  • R&D
  • Alex Genshaft
  • Mike Vilme
  • Riley Drake
  • Ira Fleming
  • Nancy Tran
  • Constantine Tzouanas
  • Jasmin Joseph-Chazan
  • Alex K. Shalek
Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
  • Genshaft et al.,
  • Nature Communications,
  • 2021
  • Genomics
  • Technology
  • R&D
  • Alex Genshaft
  • Carly Ziegler
  • Constantine Tzouanas
  • Ben Mead
  • Andrew Navia
  • Alex K. Shalek
High-fat diet-activated fatty acid oxidation mediates intestinal stemness and tumorigenicity
  • Mana et al.,
  • Cell Reports,
  • 2021
  • Biology
  • Genomics
  • Constantine Tzouanas
  • Alex K. Shalek
SARS-CoV-2 receptor ACE2 is an interferon-stimulated gene in human airway epithelial cells and is detected in specific cell subsets across tissues
  • Ziegler et al.,
  • Cell,
  • 2020
  • Biology
  • Immunology
  • Cell Atlas
  • Infectious Disease
  • Statistics
  • Genomics
  • Carly Ziegler
  • Sam Allon
  • Sarah Nyquist
  • Vincent Miao
  • Constantine Tzouanas
  • Marc Wadsworth II
  • Sam Kazer
  • Travis Hughes
  • Benjamin Doran
  • James Gatter
  • Marko Vukovic
  • Ben Mead
  • Shaina Carroll
  • Alex K. Shalek
  • José Ordovas-Montañes
SARS-CoV-2 receptor ACE2 is an interferon-stimulated gene in human airway epithelial cells and is enriched in specific cell subsets across tissues
  • Ziegler et al.,
  • SSRN,
  • 2020
  • Biology
  • Genomics
  • Infectious Disease
  • Computational Methods
  • Medicine
  • Cell Atlas
  • Immunology
  • Carly Ziegler
  • Sam Allon
  • Sarah Nyquist
  • Vincent Miao
  • Marc Wadsworth II
  • Sam Kazer
  • Travis Hughes
  • Benjamin Doran
  • James Gatter
  • Marko Vukovic
  • Constantine Tzouanas
  • Shaina Carroll
  • Alex K. Shalek
  • José Ordovas-Montañes
Spatially-resolved live cell tagging and isolation using protected photoactivatable cell dyes
  • Genshaft et al.,
  • bioRxiv,
  • 2020
  • Cancer
  • R&D
  • Technology
  • Biology
  • Genomics
  • Chemistry
  • Alex Genshaft
  • Carly Ziegler
  • Constantine Tzouanas
  • Ben Mead
  • Andrew Navia
  • Alex K. Shalek