Biography

Marc Wadsworth is a Chemistry PhD candidate in the Shalek Lab. His research interests focus on developing and implementing single-cell technologies to study infectious disease. Currently, Marc is applying these new tools to study the infectious ecosystems of M. Tuberculosis and HIV. He received his Bachelor of Science in Chemistry from Miami University in Oxford, Ohio. In addition to research, Marc enjoys teaching and is currently a teaching assistant for 5.07, Biological Chemistry I. When not in lab, he enjoys playing the piano, practicing jiu-jitsu, and cooking with his wife.

CD8+ lymphocytes are critical for early control of tuberculosis in macaques

• Winchell et al.,
• Journal of Experimental Medicine,
• 2023

• Computational Methods
• Medicine
• Infectious Disease
• Josh Bromley
• Sarah Nyquist
• Alex K. Shalek
• Marc Wadsworth II
• Travis Hughes

PI3K activation allows immune evasion by promoting an inhibitory myeloid tumor microenvironment

• Collins et al.,
• Journal for Immunotherapy of Cancer,
• 2023

• Medicine
• Biology
• Genomics
• Immunology
• Cancer
• Marc Wadsworth II
• Travis Hughes
• Alex K. Shalek

Single-cell profiling of environmental enteropathy reveals signatures of epithelial remodeling and immune activation

• Kummerlowe et al.,
• Science Translational Medicine,
• 2022

• Genomics
• Biology
• Infectious Disease
• Immunology
• Microbiology
• Cell Atlas
• Conner Kummerlowe
• Travis Hughes
• Nolawit Mulugeta
• Ira Fleming
• Marko Vukovic
• Benjamin Doran
• Toby Aicher
• Marc Wadsworth II
• José Ordovas-Montañes
• Alex K. Shalek

Multimodal profiling of lung granulomas in macaques reveals cellular correlates of tuberculosis control

• Gideon et al.,
• Immunity,
• 2022

• Biology
• Microbiology
• Infectious Disease
• Immunology
• Genomics
• Cell Atlas
• Travis Hughes
• Constantine Tzouanas
• Marc Wadsworth II
• Conner Kummerlowe
• Sarah Nyquist
• Samira Ibrahim
• José Ordovas-Montañes
• Alex K. Shalek

A single-cell liver atlas of Plasmodium vivax infection

• Mancio-Silva et al.,
• Cell Host & Microbe,
• 2022

• Microbiology
• Infectious Disease
• Genomics
• Immunology
• Biology
• Cell Atlas
• Technology
• Marc Wadsworth II
• Travis Hughes
• Alex K. Shalek

Single-cell analysis of human primary prostate cancer reveals the heterogeneity of tumor-associated epithelial cell states

• Song et al.,
• Nature Communications,
• 2022

• R&D
• Cancer
• Genomics
• Biology
• Immunology
• Marc Wadsworth II
• Alex K. Shalek

Genomic and transcriptomic correlates of immunotherapy response within the tumor microenvironment of leptomeningeal metastases

• Prakadan et al.,
• Nature Communications,
• 2021

• Biology
• Medicine
• Genomics
• Immunology
• Cancer
• Jay Prakadan
• Andrew Navia
• Kellie Kolb
• Marc Wadsworth II
• Alex K. Shalek

The cellular architecture of the antimicrobial response network in human leprosy granulomas

• Ma et al.,
• Nature Immunology,
• 2021

• Immunology
• Genomics
• Infectious Disease
• Biology
• Microbiology
• Travis Hughes
• Marc Wadsworth II
• Alex K. Shalek

Single-cell meta-analysis of SARS-CoV-2 entry genes across tissues and demographics

• Muus et al.,
• Nature Medicine,
• 2021

• Computational Methods
• Genomics
• Cell Atlas
• Statistics
• Immunology
• Infectious Disease
• Sam Allon
• Sarah Nyquist
• Marc Wadsworth II
• Carly Ziegler
• Alex K. Shalek

Single cell and spatial transcriptomics defines the cellular architecture of the antimicrobial response network in human leprosy granulomas

• Ma et al.,
• bioRxiv,
• 2020

• Genomics
• Immunology
• Infectious Disease
• Cell Atlas
• Biology
• Travis Hughes
• Marc Wadsworth II
• Alex K. Shalek

Single-cell analysis of human primary prostate cancer reveals the heterogeneity of tumor-associated epithelial cell states

• Song et al.,
• bioRxiv,
• 2020

• Biology
• Immunology
• Cancer
• Genomics
• Marc Wadsworth II
• Alex K. Shalek

Single-cell profiling of ebola virus disease in vivo reveals viral and host dynamics

• Kotliar et al.,
• Cell,

• Genomics
• Immunology
• Cell Atlas
• Infectious Disease
• Biology
• Travis Hughes
• Marc Wadsworth II
• Nancy Tran
• Alex K. Shalek

Single-cell profiling of tuberculosis lung granulomas reveals functional lymphocyte signatures of bacterial control

• Gideon et al.,
• bioRxiv,
• 2020

• Biology
• Cell Atlas
• Immunology
• Infectious Disease
• Genomics
• Travis Hughes
• Marc Wadsworth II
• Conner Kummerlowe
• Sarah Nyquist
• José Ordovas-Montañes
• Alex K. Shalek

Second-Strand Synthesis-Based Massively Parallel scRNA-Seq Reveals Cellular States and Molecular Features of Human Inflammatory Skin Pathologies

• Hughes et al.,
• Immunity,
• 2020

• Immunology
• Cell Atlas
• Genomics
• Chemistry
• Biology
• R&D
• Technology
• Travis Hughes
• Marc Wadsworth II
• José Ordovas-Montañes
• Alex K. Shalek

Single-cell profiling of Ebola virus infection ​in vivo​ reveals viral and host transcriptional dynamics

• Kotliar et al.,
• bioRxiv,
• 2020

• Infectious Disease
• Genomics
• Biology
• Immunology
• Travis Hughes
• Marc Wadsworth II
• Nancy Tran
• Alex K. Shalek

Alterations of multiple alveolar macrophage states in chronic obstructive pulmonary disease

• Bassler et al.,
• bioRxiv,
• 2020

• Immunology
• Genomics
• Medicine
• Computational Methods
• Marc Wadsworth II
• Travis Hughes
• Alex K. Shalek

SARS-CoV-2 receptor ACE2 is an interferon-stimulated gene in human airway epithelial cells and is detected in specific cell subsets across tissues

• Ziegler et al.,
• Cell,
• 2020

• Genomics
• Immunology
• Statistics
• Infectious Disease
• Biology
• Cell Atlas
• Carly Ziegler
• Sam Allon
• Sarah Nyquist
• Vincent Miao
• Constantine Tzouanas
• Marc Wadsworth II
• Sam Kazer
• Travis Hughes
• Benjamin Doran
• James Gatter
• Marko Vukovic
• Ben Mead
• Shaina Carroll
• Alex K. Shalek
• José Ordovas-Montañes

Integrated analyses of single-cell atlases reveal age, gender, and smoking status associations with cell type-specific expression of mediators of SARS-CoV-2 viral entry and highlights inflammatory programs in putative target cells

• Muus et al.,
• bioRxiv,

• Cell Atlas
• Infectious Disease
• Biology
• Computational Methods
• Genomics
• Immunology
• Statistics
• Sam Allon
• Sarah Nyquist
• José Ordovas-Montañes
• Marc Wadsworth II
• Carly Ziegler
• Alex K. Shalek

Systematic comparison of single-cell and single-nucleus RNA-sequencing methods

• Ding et al.,
• Nature Biotechnology,
• 2020

• Computational Methods
• Technology
• R&D
• Genomics
• Travis Hughes
• Marc Wadsworth II
• Shaina Carroll
• Alex K. Shalek

SARS-CoV-2 receptor ACE2 is an interferon-stimulated gene in human airway epithelial cells and is enriched in specific cell subsets across tissues

• Ziegler et al.,
• SSRN,
• 2020

• Medicine
• Biology
• Computational Methods
• Immunology
• Genomics
• Infectious Disease
• Cell Atlas
• Carly Ziegler
• Sam Allon
• Sarah Nyquist
• Vincent Miao
• Marc Wadsworth II
• Sam Kazer
• Travis Hughes
• Benjamin Doran
• James Gatter
• Marko Vukovic
• Constantine Tzouanas
• Shaina Carroll
• Alex K. Shalek
• José Ordovas-Montañes

Identification of a master regulator of differentiation in toxoplasma

• Waldman et al.,
• Cell,
• 2020

• Infectious Disease
• Immunology
• Genomics
• Microbiology
• Biology
• Marc Wadsworth II
• Alex K. Shalek

Prevention of tuberculosis in macaques after intravenous BCG immunization

• Darrah et al.,
• Nature,
• 2020

• Infectious Disease
• Medicine
• Immunology
• Genomics
• Biology
• Marc Wadsworth II
• Travis Hughes
• Alex K. Shalek

Loss of DNA methyltransferase activity in primed human ES cells triggers increased cell-cell variability and transcriptional repression

• Tsankov et al.,
• Development,
• 2019

• Genomics
• Biology
• Marc Wadsworth II
• Sam Allon
• Ben Mead
• Riley Drake
• Alex K. Shalek

Highly efficient, massively-parallel single-cell RNA-Seq reveals cellular states and molecular features of human skin pathology

• Hughes T.K. et al.,
• bioRxiv,
• 2019

• Genomics
• R&D
• Immunology
• Biology
• Technology
• Travis Hughes
• Marc Wadsworth II
• José Ordovas-Montañes
• Alex K. Shalek

Identification of a Master Regulator of Differentiation in Toxoplasma

• Waldman B.S. et al.,
• bioRxiv,
• 2019

• Biology
• Microbiology
• Infectious Disease
• Marc Wadsworth II
• Alex K. Shalek

Seq-Well: A Sample-Efficient, Portable Picowell Platform for Massively Parallel Single-Cell RNA Sequencing

• Aicher et al.,
• Single-Cell Methods,
• 2019

• R&D
• Technology
• Toby Aicher
• Shaina Carroll
• Marc Wadsworth II
• Travis Hughes
• Alex K. Shalek

Single-Cell RNA-Seq Reveals AML Hierarchies Relevant to Disease Progression and Immunity

• van Galen et al.,
• Cell,
• 2019

• Marc Wadsworth II
• Travis Hughes
• Alex K. Shalek

Allergic Inflammatory Memory in Human Respiratory Epithelial Progenitor Cells

• Ordovas-Montanes et al.,
• Nature,
• 2018

• Medicine
• Biology
• Immunology
• Cell Atlas
• José Ordovas-Montañes
• Sarah Nyquist
• Marko Vukovic
• Marc Wadsworth II
• Travis Hughes
• Sam Kazer
• Alex K. Shalek

Harnessing single-cell genomics to improve the physiological fidelity of organoid-derived cell types

• Mead B.E.* et al.,
• BMC Biology,
• 2018

• Ben Mead
• José Ordovas-Montañes
• Travis Hughes
• Marc Wadsworth II
• Alex K. Shalek

Reduced cellular diversity and an altered basal progenitor cell state inform epithelial barrier dysfunction in human type 2 immunity

• Jose Ordovas-Montanes et al.,
• BioRxiv,
• 2017

• Medicine
• Cell Atlas
• Immunology
• José Ordovas-Montañes
• Sarah Nyquist
• Marc Wadsworth II
• Travis Hughes
• Sam Kazer
• Alex K. Shalek

Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput

• Gierahn et al.,
• Nature Methods,
• 2017

• Chemistry
• R&D
• Technology
• Travel
• Genomics
• Marc Wadsworth II
• Travis Hughes
• Alex K. Shalek

Dissecting the multicellular ecosystem of metastatic melanoma by single-cell RNA-seq

• Tirosh et al.,
• Science,
• 2016

• Medicine
• Cancer
• Biology
• Genomics
• Jay Prakadan
• Marc Wadsworth II
• Alex Genshaft
• Travis Hughes
• Carly Ziegler
• Sam Kazer
• Aleth Gaillard
• Kellie Kolb
• Alex K. Shalek

Marrying microfluidics and microwells for parallel, high-throughput single-cell genomics

• Wadsworth II et al.,
• Genome Biology,
• 2015

• Technology
• Genomics
• R&D
• Marc Wadsworth II
• Travis Hughes
• Alex K. Shalek