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Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput

Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput

Seq-Well is a portable, low-cost platform for single-cell RNA sequencing designed to be compatible with low-input, clinical biopsies. We have recently published a manuscript detailing the development and validation of the Seq-Well plaftorm. Here, we provide an in-depth protocol and videos describing how to perform Seq-Well experiments.

We hope that you will use Seq-Well and apply it in your work.

CAD Files: 

CAD files for Seq-well devices and molds are provided here: CAD Files

Note: These files are made available under the “Attribution-NonCommercial-NoDerivatives 4.0 International” creative commons license.

Copyright 2017, Massachusetts Institute of Technology and the Broad Institute.

Publications, Protocols and Additional Resources

Gierahn, T.M*, Wadsworth II, M.H.*, Hughes, T.K.*, Bryson, B.D, Butler, A, Satija, R, Fortune, S, Love, J.C.†, Shalek, A.K.†, Nature Methods Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput
Technology
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Protocol: Hughes.et_.al_.Master.Protocol.pdf
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