Probing Enzymatic Activity inside Living Cells Using a Nanowire−Cell “Sandwich” Assay

  • Biology
  • Chemistry
  • Immunology
  • Technology
  • Alex K. Shalek
  • Na et al.▾
    Na, Y.R., Kim, S.Y., Gaublomme, J.T., Shalek, A.K., Jorgollia, M., Park, H. and Yang, E.G.
  • Nano Letters , Volume 13 , Issue 153
  • January, 2013
Biology
Chemistry
Immunology
Technology
Alex K. Shalek

Abstract

Developing a detailed understanding of enzyme function in the context of an intracellular signal transduction pathway requires minimally invasive methods for probing enzyme activity in situ. Here, we describe a new method for monitoring enzyme activity in living cells by sandwiching live cells between two vertical silicon nanowire (NW) arrays. Specifically, we use the first NW array to immobilize the cells and then present enzymatic substrates intracellularly via the second NW array by utilizing the NWs’ ability to penetrate cellular membranes without affecting cells’ viability or function. This strategy, when coupled with fluorescence microscopy and mass spectrometry, enables intracellular examination of protease, phosphatase, and protein kinase activities, demonstrating the assay’s potential in uncovering the physiological roles of various enzymes.

Probing Enzymatic Activity inside Living Cells Using a Nanowire−Cell “Sandwich” Assay