Scaling by shrinking: empowering single-cell 'omics' with microfluidic devices

Genomics Genomics
Technology Technology
Alex K. Shalek Alex K. Shalek
Jay Prakadan Jay Prakadan

Prakadan et al.▾ Prakadan, S.M., Shalek, A.K., Weitz, D.A.

Nature Reviews Genetics

April, 2017


Recent advances in cellular profiling have demonstrated substantial heterogeneity in the behaviour of cells once deemed 'identical', challenging fundamental notions of cell 'type' and 'state'. Not surprisingly, these findings have elicited substantial interest in deeply characterizing the diversity, interrelationships and plasticity among cellular phenotypes. To explore these questions, experimental platforms are needed that can extensively and controllably profile many individual cells. Here, microfluidic structures — whether valve-, droplet- or nanowell-based — have an important role because they can facilitate easy capture and processing of single cells and their components, reducing labour and costs relative to conventional plate-based methods while also improving consistency. In this article, we review the current state-of-the-art methodologies with respect to microfluidics for mammalian single-cell 'omics' and discuss challenges and future opportunities.