Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput
Chemistry
Genomics
R&D
Technology
Travel
Alex K. Shalek
Marc Wadsworth II
Travis Hughes
Nature Methods , Volume 14
March, 2017
Abstract
Single-cell RNA-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA-seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We use Seq-Well to profile thousands of primary human macrophages exposed to Mycobacterium tuberculosis.