By affinity capture and amplification of TCR transcripts from whole-transcriptome libraries, TCR CDR3 sequences can be recovered from 3′-barcoded scRNA-seq libraries (e.g. Seq-Well, Drop-seq, etc.). This method can be applied post-hoc, allowing for the capture of additional information from archived samples. The protocol can also be found here.

Seq-Well TCR Enrichment

Publications, Protocols and Additional Resources

Lab Members Involved

  • Alex K. Shalek

Research Areas

  • Biology
  • Cell Atlas
  • Genomics
  • Immunology
  • R&D
  • Technology