Seq-Well is a portable, low-cost platform for single-cell RNA sequencing designed to be compatible with low-input, clinical biopsies. We have recently published a manuscript detailing the development and validation of the Seq-Well plaftorm. Here, we provide an in-depth protocol and videos describing how to perform Seq-Well experiments.

We hope that you will use Seq-Well and apply it in your work.

Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput
  • Gierahn et al.,
  • Nature Methods,
  • 2017
  • Travel
  • Genomics
  • R&D
  • Chemistry
  • Technology
  • Marc Wadsworth
  • Travis Hughes
  • Alex K. Shalek

 

 

CAD Files: 

CAD files for Seq-well devices and molds are provided here: CAD Files

Note: These files are made available under the “Attribution-NonCommercial-NoDerivatives 4.0 International” creative commons license.

Copyright 2017, Massachusetts Institute of Technology and the Broad Institute.

Seq-Well

Publications, Protocols and Additional Resources

Lab Members Involved

  • Marc Wadsworth
  • Travis Hughes
  • Alex K. Shalek

Research Areas

  • Cancer
  • Chemistry