High-throughput phenotypic screens using biochemical perturbations
and high-content readouts are constrained by limitations of scale. To
address this, we establish a method of pooling exogenous perturbations
followed by computational deconvolution to reduce required sample size,
labor and cost. We demonstrate the increased efficiency of compressed
experimental designs compared to conventional approaches through
benchmarking with a bioactive small-molecule library and a high-content
imaging readout.
Th1 CD4+ T cells mediate protective antiMtb immunity across biological systems
and organisms. Bromley, Ganchua, et al.
demonstrate that CD4+ T cells regulate
immune tone in TB granulomas and are
necessary for immune recall and
protection against reinfection. Following
reinfection, CD4+ T cells facilitate the
development of a growth restrictive niche
via the induction of immuno-modulatory
genes and cellular interaction networks.
Recent case reports and epidemiological data suggest that fungal infections represent an underappreciated complication among people with severe COVID-19. However, the frequency of fungal colonization in patients with COVID-19 and associations with specific immune responses in the airways remain incompletely defined.