We combine genomics, chemical biology, and nanotechnology to construct accessible and widely-useful cross-disciplinary platforms that enable us and others to profile and control cells and their interactions. By applying these approaches with partners around the world, we can identify and test how cellular composition and communication drive successful or aberrant ensemble immune responses and influence homeostatic stability to environmental perturbations across organs and diseases. We are committed to sharing and training others in these technologies, both to empower global inquiry and to discover limitations to overcome.

Identities

We develop single-cell transcriptomic approaches to comprehensively profile human tissues and model systems. Previously, we focused on establishingvalidating, scaling, and simplifying single-cell RNA-seq, often through the development of microdevices, to enable genome-wide identification of the cell types/states contained within complex biological samples. More recently, we helped both enhance the detection of phenotype-defining transcripts using these methods and simplify their on-site processing for clinical applications. In parallel, we have also worked to democratize these techniques, providing open access to resources and protocols, training thousands locally and abroad, and establishing infrastructure and on-site collaborations spanning across 6 continents and 26+ countries.

Identities

Lab Members Involved

Alex K. Shalek Alex K. Shalek
Andrew Navia Andrew Navia
Brittany Goods Brittany Goods
Carly Ziegler Carly Ziegler
Jay Prakadan Jay Prakadan
José Ordovas-Montañes José Ordovas-Montañes
Marc Wadsworth II Marc Wadsworth II
Sam Kazer Sam Kazer
Travis Hughes Travis Hughes
Vincent Miao Vincent Miao

Research Areas

Biology Biology
Cancer Cancer
Cell Atlas Cell Atlas
Chemistry Chemistry
Computational Methods Computational Methods
Genomics Genomics
Immunology Immunology
Infectious Disease Infectious Disease
Physics Physics
R&D R&D
Statistics Statistics
Technology Technology

Characteristics

As many factors define cellular phenotype and influence disease beyond mRNA, we develop complementary methods for co-assaying other cellular attributes to enrich our understanding of the drivers of cellular behaviors. Examples including the abundance of additional ‘-omes’, the sequence and amount of important transcripts, cellular history, biophysical properties, spatial position, and functional output. Recently, we have worked to: 1. detect pathogens in cells and potentially actionable associated host factors; 2. query for specific mutations to identify cancer cells; and, 3. extract T cell receptor sequences to examine clonality. We have also formulated computational methods to derive deeper insights from these data (e.g., to examine viral dynamic in infected cells, reproducible features hidden by inter-individual variability, multicellular immune dynamics, intercellular communication, or alteration in cellular ecosystems associated with pathology).

Characteristics

Lab Members Involved

Aleth Gaillard Aleth Gaillard
Alex Genshaft Alex Genshaft
Alex K. Shalek Alex K. Shalek
Carly Ziegler Carly Ziegler
Jay Prakadan Jay Prakadan
Marc Wadsworth II Marc Wadsworth II
Sam Allon Sam Allon
Sam Kazer Sam Kazer
Travis Hughes Travis Hughes
Vincent Miao Vincent Miao

Research Areas

Biology Biology
Cancer Cancer
Chemistry Chemistry
Computational Methods Computational Methods
Genomics Genomics
Immunology Immunology
Infectious Disease Infectious Disease
Microbiology Microbiology
Physics Physics
R&D R&D
Statistics Statistics
Technology Technology

Environments

We explore how the extracellular milieu influences cellular decision-making. Here, we have employed controlled culture conditions with cells and organoids, chemical and genetic perturbations, and constant microfluidic perfusion. We also have leveraged natural microenvironmental variation within and across tissues via microdissection and by using photoactivatable probes that retain spatial information through dissociation. In each instance, we aim to understand the degree to which extracellular environments modulate, and can be used to rationally control, the responses of individual cells or the overall distribution thereof, with an eye toward engineering tissue responses.

Environments

Lab Members Involved

Ben Mead Ben Mead
Carly Ziegler Carly Ziegler
Constantine Tzouanas Constantine Tzouanas
Jennyfer Galvez-Reyes Jennyfer Galvez-Reyes
Josh Bromley Josh Bromley
Nolawit Mulugeta Nolawit Mulugeta
Peter Winter Peter Winter
Riley Drake Riley Drake
Sam Allon Sam Allon
Vincent Miao Vincent Miao

Research Areas

Biology Biology
Cancer Cancer
Chemistry Chemistry
Computational Methods Computational Methods
Immunology Immunology
Infectious Disease Infectious Disease
Microbiology Microbiology
Physics Physics
R&D R&D
Statistics Statistics
Technology Technology

Interactions

We examine the impact of intercellular interactions on cellular function. We have used coculture, imaging and perturbation strategies, as well as matched computational methods, to reinforce findings from dissociated samples, validate inferred cell-cell communication in vivo (e.g., between sensory neurons and lymph node resident cells), and manipulate multicellular systems (e.g., organoids). We are currently working on building arrayed, synthetically-designed cellular ensembles to examine how ‘tissue’ structure impacts functional response. Our overall goal is to understand cellular co-dependencies that influence niche- and tissue-level response dynamics.

Interactions

Lab Members Involved

Alex Genshaft Alex Genshaft
Andrew Navia Andrew Navia
Brittany Goods Brittany Goods
Carly Ziegler Carly Ziegler
Jay Prakadan Jay Prakadan
José Ordovas-Montañes José Ordovas-Montañes
Kellie Kolb Kellie Kolb
Marc Wadsworth II Marc Wadsworth II
Marko Vukovic Marko Vukovic
Sarah Nyquist Sarah Nyquist

Research Areas

Biology Biology
Cancer Cancer
Chemistry Chemistry
Computational Methods Computational Methods
Genomics Genomics
Immunology Immunology
Infectious Disease Infectious Disease
Microbiology Microbiology
Physics Physics
R&D R&D
Statistics Statistics
Technology Technology

Integrations

We broadly study how intra- and extracellular circuits collectively drive healthy and diseased tissue states. By leveraging the massive genomic datasets we and others have generated from complex tissues (like melanoma tumors, inflamed gut, and nasal polyps), we have begun to identify common and unique cell types/states and circuits associated with pathology that may be important for regulating biological function and stability. Our current findings suggest multiple overlaps among distinct diseases, pointing to the possibility of a finite set of evolved response strategies and thus common interventions based on adjusting specific cell states, cell frequencies, and/or cell-cell communication pathways.

Integrations

Lab Members Involved

Ben Mead Ben Mead
Benjamin Doran Benjamin Doran
Conner Kummerlowe Conner Kummerlowe
Constantine Tzouanas Constantine Tzouanas
Jay Prakadan Jay Prakadan
Marc Wadsworth II Marc Wadsworth II
Marko Vukovic Marko Vukovic
Samira Ibrahim Samira Ibrahim
Sarah Nyquist Sarah Nyquist
Vincent Miao Vincent Miao

Research Areas

Biology Biology
Cancer Cancer
Cell Atlas Cell Atlas
Computational Methods Computational Methods
Genomics Genomics
Immunology Immunology
Medicine Medicine
R&D R&D
Statistics Statistics