Our recent paper details a strategy, co-developed with the Love Lab at MIT, for pairing TCR sequencing with whole-transcriptome profiling in single-cells via 3'-barcoded techniques. Using a targeted pulldown and amplification method, we are able to sequence TCR CDR3 regions from pre-existing scRNA-seq libraries, increasing the amount of orthogonal information…

We’re stoked to have this paper up! We present our work finding new roles for sensory neurons within inflammatory responses and previously unappreciated axes of neuro-immune crosstalk. Led by Shalek lab MD-PhD student Carly Ziegler and post doc Tracy Huang with Uli von Andrian, we find that lymph nodes are…

Alex was recently featured in Nature Methods to discuss challenges and future opportunities in high-throughput genomics techniques. He joins researchers spanning across the spectrum of biology disciplines in laying out technological challenges of their respective fields and the great potential next-generation techniques hold in better understanding complex biological systems. Read…