Our recent paper details a strategy, co-developed with the Love Lab at MIT, for pairing TCR sequencing with whole-transcriptome profiling in single-cells via 3′-barcoded techniques. Using a targeted pulldown and amplification method, we are able to sequence TCR CDR3 regions from pre-existing scRNA-seq libraries, increasing the amount of orthogonal information recoverable from archived samples. Read press releases on the technique here and here, as well as the protocol and computational tools.
New Paper on Neuroimmune Interactions on BioRxivCarly’s Visualizations Selected as One of 2019’s Best Science Images by the Nature News Team