The Shalek Lab strives to engineer and create technologies and methods achievable by any lab, anywhere in the world. See our open source experimental protocols, computational packages, and favorite recipes below.

Mapping Allergic Inflammation

Download Raw Data Download Metadata scRNA-seq data captured on Seq-Well for the core 18,036 cell data set as clustered and analyzed in Figure 1 of  “Allergic inflammatory memory in human respiratory epithelial progenitor cells“. Raw cell (columns) x gene (rows)...

scRAD: Single-Cell Reproducibility Across Donors

scRAD: Single-Cell Reproducibility Across Donors scRAD supports a reproducibility-driven framework for assessing the expression differences in single-cell RNA-Seq (scRNA-Seq) data collected from multiple donors. scRAD provides tools for: Reproducible gene module analysis – identifying axes of variation common across donors Irreproducible...

Spicy Mezcal

In the Shalek Lab, we bring the same level of commitment and attention to detail we apply to our science to our cooking and cocktails. Here are recipes for 3 pepper-infused mezcals, ranked X, XX and XXX based on their...

Seq-Well

Seq-Well is a portable, low-cost platform for single-cell RNA sequencing designed to be compatible with low-input, clinical biopsies. We have recently published a manuscript detailing the development and validation of the Seq-Well plaftorm. Here, we provide an in-depth protocol and videos describing...